Discovery HS F5 HPLC Column, 3 mum particle size, L x I.D. 15 cm x 4.6 mm en Nottingham, Inglaterra, Reino Unido
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Especificaciones
- Condición
- nuevo
- Número de stock
- 567507-U
- Agency
- suitable for USP L43
- Application(s)
- food and beverages
- Extent of labeling
- 12% Carbon loading
- Feature
- endcapped
- Impurities
- ﹤10 ppm metals
- L × i.d.
- 15 cm × 4.6 mm
- Manufacturer/tradename
- Discovery®
- Material
- stainless steel column
- Matrix active group
- PFP (pentafluorophenyl) phase
- Matrix
- fully porous particle, silica gel, high purity, spherical particle platform
- Operating ph range
- 2-8
- Packaging
- 1 ea of
- Parameter
- ≤70 °C temp. range, 400 bar pressure (5801 psi)
- Particle size
- 3 µm
- Pore size
- 120 Å
- Product line
- Discovery®
- Quality level
- 100
- Separation technique
- reversed phase
- Surface area
- 300 m2/g
- Surface coverage
- 4 µmol/m2
- Technique(s)
- HPLC: suitable, LC/MS: suitable
- Categoría
- Cromatografía en Reino Unido
- Subcategoría
- Hplc
- ID de Anuncio
- 73784814
Descripción
Features and Benefits
Unique selectivity Similar retention to C18 (sometimes requires stronger mobile phase) Excellent peak shape Stable, low-bleed LC-MS separations Scalable separations from 3 to 10µm particle sizes
General description
Guidelines for transferring a C18 method to Discovery® HS F5:Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
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